منابع مشابه
Dual Fluorescence from N6,N6-Dimethyladenosine
The adenosine derivative N6,N6-dimethyladenosine (DMA) shows dual fluorescence in solvents of different polarity. In addition to the “normal” fluorescence at 330 nm, another band is observed at 500 nm. The long wavelength emission dominates in aprotic solvents but is dynamically quenched by protic solvents. Steady-state and lifetime measurements show that the emissions originate from two excite...
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High hydrostatic pressure affects in various ways the reactivity and the stability of biogenic macromolecules [ 11. Pressures of several hundred bars reduce or increase enzymic activity [2-41. Proteins are usually irreversibly denaturated by pressures in excess of 1 kbar. On the other hand, calf thymus DNA is stabilized by increased pressure [5]. In general, an increase in pressure shifts an eq...
متن کامل1895-1900 Ma LAJP 2627:Ma
A sensitive and selective liquid chromatography tandem mass spectrometry (LC–MS/MS) method for determination of meropenem in rabbit plasma was developed. After addition of triazolam as internal standard (IS), protein precipitation by acetonitrile was used in sample preparation. Chromatographic separation was achieved on a Restek Allure (TM) PFP Propyl (2.1 mm × 100 mm, 5 μm) column with acetoni...
متن کامل292-296 Ma LAJP 3128:Ma
A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-MS/MS) method for determination of gefitinib in rat plasma using one-step protein precipitation was developed. After addition of estazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 mm × 50 mm, 3.5 μm) col...
متن کاملBarrier-to-autointegration factor (BAF) condenses DNA by looping.
Barrier-to-autointegration factor (BAF) is a protein that has been proposed to compact retroviral DNA, making it inaccessible as a target for self-destructive integration into itself (autointegration). BAF also plays an important role in nuclear organization. We studied the mechanism of DNA condensation by BAF using total internal reflection fluorescence microscopy. We found that BAF compacts D...
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ژورنال
عنوان ژورنال: Nature Chemical Biology
سال: 2018
ISSN: 1552-4450,1552-4469
DOI: 10.1038/s41589-018-0199-9